Conditional knockout of its mammalian homolog Piezo1 in vivo accelerates regeneration, while its pharmacological activation in vitro modestly reduces regeneration, suggesting the role of Piezo in inhibiting regeneration may be evolutionarily conserved. Loss of DmPiezo enhances axon regeneration of sensory neurons in the peripheral and CNS. DmPiezo activation during axon regeneration induces local Ca 2+ transients at the growth cone, leading to activation of nitric oxide synthase and the downstream cGMP kinase Foraging or PKG to restrict axon regrowth. Here, we show that DmPiezo, a Ca 2+-permeable non-selective cation channel, functions as an intrinsic inhibitor for axon regeneration in Drosophila. Given that mechanical forces affect neuronal outgrowth, it is important to investigate whether mechanosensitive ion channels may regulate axon regeneration. Electronic address: exhibit a limited ability of repair.

11 Departments of Physiology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.

10 Departments of Physiology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.

9 Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.

8 Department of Neuroscience, The Scripps Research Institute, Howard Hughes Medical Institute, La Jolla, CA 92037, USA.

7 Department of Neurobiology, University of Massachusetts Medical School, Worcester, MA 01605, USA.

Perelman Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.

5 Departments of Physiology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA.

4 The Graduate Group in Biochemistry and Molecular Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA.3 Department of Dermatology, University of Pennsylvania, Philadelphia, PA 19104, USA.Perelman Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Nippon zebra 2 4 piezo